1GTT
CRYSTAL STRUCTURE OF HPCE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 126.084, 138.201, 103.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.290 - 1.700 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.25200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.023 |
| Data reduction software | MAR |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.06) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.055 | 0.546 |
| Number of reflections | 196319 | |
| <I/σ(I)> | 18.6 | 2.2 |
| Completeness [%] | 98.8 | 97.5 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | MONOMETHYLETHER PEG2000, CALCIUM CHLORIDE, TRIS, pH 8.00 |
