1GTR
STRUCTURAL BASIS OF ANTICODON LOOP RECOGNITION BY GLUTAMINYL-TRNA SYNTHETASE
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 242.800, 94.300, 115.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 3.200 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Number of reflections | 37820 |
| Completeness [%] | 84.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 17 * | referred to J.Mol.Biol. 202.121 1988 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PIPES | 80 (mM) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | ATP | 4 (mM) | |
| 4 | 1 | reservoir | beta-mercaptoethanol | 20 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | drop | tRNA enzyme |
