1GTP
GTP CYCLOHYDROLASE I
Experimental procedure
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 204.200, 210.400, 71.800 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.000 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.490 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 3.000 | |
| Rmerge | 0.113 | |
| Total number of observations | 209458 * | |
| Number of reflections | 111785 | |
| Completeness [%] | 93.2 | 84 * |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | ||
| 2 | 1 | drop | K,Na-phosphate | 20 (mM) | |
| 3 | 1 | drop | EDTA | 5 (mM) | |
| 4 | 1 | reservoir | Na-citrate | 0.5 (M) |
