1GSX
CRYSTAL STRUCTURE OF THE P65 CRYSTAL FORM OF PHOTOACTIVE YELLOW PROTEIN G47S/G51S MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 40.420, 40.420, 118.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.790 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d7e |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.490 * |
| Data reduction software | MAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.069 | 0.523 |
| Total number of observations | 26058 * | |
| Number of reflections | 9953 * | 936 * |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 95.8 | 91.7 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | PEG 2K, MES., pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | phosphate | 50 (mM) | pH7. |
| 2 | 1 | drop | protein | 25 (mg/ml) | |
| 3 | 1 | reservoir | PEG2000MME | ||
| 4 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
