1GSS

THREE-DIMENSIONAL STRUCTURE OF CLASS PI GLUTATHIONE S-TRANSFERASE FROM HUMAN PLACENTA IN COMPLEX WITH S-HEXYLGLUTATHIONE AT 2.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	60.500, 60.500, 238.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.196
RMSD bond length	0.015
RMSD bond angle	3.445
Phasing software	X-PLOR
Refinement software	EREF

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.800 *
Rmerge	0.103 *
Total number of observations	26034 *
Number of reflections	11181 *
Completeness [%]	94.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium phosphate	10 (mM)
3	1	drop	ammonium sulfate	45-55 (%sat)
4	1	drop	sodium phosphate	100 (mM)
5	1	drop	S-hexylglutathione	10 (mM)
6	1	reservoir	ammonium sulfate	45-55 (%sat)
7	1	reservoir	sodium phosphate	100 (mM)
8	1	reservoir	S-hexylglutathione	10 (mM)