1GSO
GLYCINAMIDE RIBONUCLEOTIDE SYNTHETASE (GAR-SYN) FROM E. COLI.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1997-08-01 |
| Detector | ADSC |
| Wavelength(s) | 0.979419, 0.979104, 0.967642, 0.9190 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.220, 62.420, 129.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.043 * | 0.174 * |
| Number of reflections | 58385 | 7256 * |
| <I/σ(I)> | 10 | 4.1 |
| Completeness [%] | 95.9 | 83.9 |
| Redundancy | 5.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 18 * | 0.1M MES PH6.3, 0.2M AMMONIUM SULFATE, 26% PEG 5K MONOMETHYLETHER, HANGING DROP VAPOR DEFFUSION, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 0.1 (M) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG MME5000 | 24 (%) |
