1GSG

Structure of E.coli glutaminyl-tRNA synthetase complexed with trnagln and ATP at 2.8 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	C 2 2 21
Unit cell lengths	242.800, 93.600, 115.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.800
R-factor	0.279
Rwork	0.279
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	18.000 *
High resolution limit [Å]	2.800
Rmerge	0.044 *
Total number of observations	152900 *
Number of reflections	31768 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		17 *	referred to J.Mol.Biol. 202.121 1988 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PIPES	80 (mM)
2	1	reservoir		20 (mM)
3	1	reservoir	ATP	4 (mM)
4	1	reservoir	beta-mercaptoethanol	20 (mM)
5	1	drop	tRNA enzyme
6	1	reservoir	ammonium sulfate	2.0 (M)