1GSE
GLUTATHIONE TRANSFERASE A1-1 COMPLEXED WITH AN ETHACRYNIC ACID GLUTATHIONE CONJUGATE (MUTANT R15K)
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-01-20 |
Detector | RIGAKU RAXIS |
Spacegroup name | C 1 2 1 |
格子定数 [Å] | 100.400, 94.800, 51.800 |
格子定数 [度] | 90.00, 92.96, 90.00 |
精密化法
残基 | 7.500 - 2.000 |
R因子 | 0.197 |
Rwork | 0.197 |
R-free | 0.24400 * |
結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
結合角の平均二乗偏差(RMSD) [度] | 1.440 * |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Quality characteristics
Overall | |
分解能 [Å] (低) | 15.000 |
分解能 [Å] (高) | 2.000 |
Rmerge_l_obs | 0.073 |
Total number of observations | 64609 * |
独立反射数 | 30538 |
完全性 [%] | 93.4 |
冗長性 | 2.1 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion * | 20 * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 55 (mg/ml) | |
2 | 1 | drop | PEG2000 | 10-20 (%) | |
3 | 1 | drop | Tris-HCl | 100 (mM) | |
4 | 1 | drop | NaAc | 30 (mM) | |
5 | 1 | drop | beta-mercaptoethanol | 1 (%) | |
6 | 1 | reservoir | PEG2000 | 10-20 (%) | |
7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
8 | 1 | reservoir | NaAc | 30 (mM) | |
9 | 1 | reservoir | beta-mercaptoethanol | 1 (%) |