1GS9

Apolipoprotein E4, 22k domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	125
Detector technology	CCD
Collection date	1999-04-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	45.511, 53.089, 73.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.900 - 1.700
R-factor	0.219
Rwork	0.218
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bz4
RMSD bond length	0.016
RMSD bond angle	1.794
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	EPMR
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.900
High resolution limit [Å]	1.700
Number of reflections	20168
<I/σ(I)>	12.1
Completeness [%]	100.0
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		50MM NA-CACODYLATE, PH 5.6, 20-25% PEG 400, 1% 2-ME, RT, CRYSTALLIZED FROM FULL LENGTH APOE4 CONSTRUCT (299 RESIDUES). PROTEOLYTIC CLEAVAGE IN CRYSTALLIZATION DROP TO 22K FRAGMENT. NEW, THIRD ORTHOGONAL CRYSTAL FORM OF APOE (ORTHO-3)