1GRZ
A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRANDEIS |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 179.000, 179.000, 199.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 5.000 |
| R-factor | 0.3686 |
| Rwork | 0.369 |
| R-free | 0.42850 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.240 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 5.180 |
| High resolution limit [Å] | 5.000 | 5.000 |
| Rmerge | 0.052 | 0.319 |
| Number of reflections | 14377 | |
| <I/σ(I)> | 27 | 10 |
| Completeness [%] | 99.3 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 10 (mg/ml) | |
| 2 | 1 | reservoir | 10 (mM) | ||
| 3 | 1 | reservoir | potassium caodylate | 50 (mM) | |
| 4 | 1 | reservoir | 50 (mM) | ||
| 5 | 1 | reservoir | spermine | 0.5 (mM) | |
| 6 | 1 | reservoir | MPD | 0.5 (mM) |
