1GRO
REGULATORY AND CATALYTIC MECHANISMS IN ESCHERICHIA COLI ISOCITRATE DEHYDROGENASE: MULTIPLE ROLES FOR N115
Experimental procedure
Detector technology | IMAGE PLATE |
Detector | RAXIS |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 105.100, 105.100, 150.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.500 |
R-factor | 0.195 |
Rwork | 0.195 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.800 |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | |
High resolution limit [Å] | 2.500 | 2.500 * |
Rmerge | 0.099 | 0.265 * |
Number of reflections | 26055 | 1921 * |
Completeness [%] | 86.7 | 65.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | dephosphorylated IDH | 28 (mg/ml) | |
2 | 1 | 1 | ammonium sulfate | 34 (%sat) | |
3 | 1 | 1 | 100 (mM) | ||
4 | 1 | 1 | 35 (mM) | ||
5 | 1 | 1 | citric acid | 9 (mM) | |
6 | 1 | 1 | dithiothreitol | 0.2 (mM) |