1GQZ
Refinement of Haemophilus influenzae Diaminopimelate epimerase at 1.7A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 290 |
| Collection date | 1999-07-15 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.637, 113.870, 64.481 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bwz |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 * | 2.000 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.039 | 0.212 |
| Number of reflections | 37359 * | |
| <I/σ(I)> | 29.98 | 7.41 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 290 * | 0.1M IMIDIZOLE PH 6.5, |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 1 (M) | |
| 2 | 1 | drop | imidazole | 0.1 (M) | pH6.5 |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | HEPES | 25 (mM) | pH8.0 |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) |
