1GQW
Taurine/alpha-ketoglutarate Dioxygenase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-12-15 |
Detector | MARRESEARCH |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 116.850, 116.850, 201.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.440 - 3.000 |
R-factor | 0.281 |
Rwork | 0.281 |
R-free | 0.32000 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 24.600 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.490 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.065 | 0.270 |
Total number of observations | 98529 * | |
Number of reflections | 16727 * | |
<I/σ(I)> | 10.5 | 2.8 |
Completeness [%] | 99.3 | 99.8 |
Redundancy | 5.9 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 17 * | 20-28% PEG1000, 20% ETHYLENE GLYCOL, 75MM IMIDAZOLE PH7.5, PROTEIN SOLUTION LOADED WITH FE(II), ALPHA-KETOGLUTARATE, TAURINE, DITHIOTHREITOL, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH7.0 |
3 | 1 | drop | alphaKG | 200 (mM) | |
4 | 1 | drop | taurine | 200 (mM) | pH7.0 |
5 | 1 | drop | dithiothreitol | 100 (mM) | |
6 | 1 | reservoir | PEG1000 | 20-28 (%) | |
7 | 1 | reservoir | imidazole | 75 (mM) | pH7.0 |
8 | 1 | reservoir | ethylene glycol | 20 (%) |