1GQQ
MURC - Crystal structure of the apo-enzyme from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.508, 99.485, 180.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 3.100 |
| R-factor | 0.24134 |
| Rwork | 0.239 |
| R-free | 0.28624 |
| Structure solution method | MIR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.051 | 0.396 |
| Number of reflections | 20892 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.6 | PROTEIN SOLUTION 12MG/ML CONTAINING 10% GLYCEROL MIXED WITH WELL SOLUTION CONTAINING 2.4M AMMONIUM SULPHATE, 100MM BICINE BUFFER AND 3%ETHANOL., pH 8.60 |
