1GQO
Type II Dehydroquinase from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.210, 195.610, 97.360 |
Unit cell angles | 90.00, 91.87, 90.00 |
Refinement procedure
Resolution | 21.840 - 2.100 |
Rwork | 0.201 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STREPTOMYCES COELICOLOR DODECAMER |
RMSD bond length | 0.016 |
RMSD bond angle | 0.039 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.840 | 2.400 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.102 | |
Number of reflections | 174109 | |
<I/σ(I)> | 1 | |
Completeness [%] | 82.5 | 64.2 |
Redundancy | 2.9 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | 5% PEG8K, 0.05M CACL2, 0.1M TRIS PH 8.5 |