1GQO

Type II Dehydroquinase from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-15
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	97.210, 195.610, 97.360
Unit cell angles	90.00, 91.87, 90.00

Refinement procedure

Resolution	21.840 - 2.100
Rwork	0.201
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	STREPTOMYCES COELICOLOR DODECAMER
RMSD bond length	0.016
RMSD bond angle	0.039
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.840	2.400
High resolution limit [Å]	2.100	2.100
Rmerge	0.102
Number of reflections	174109
<I/σ(I)>		1
Completeness [%]	82.5	64.2
Redundancy	2.9	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.2		5% PEG8K, 0.05M CACL2, 0.1M TRIS PH 8.5