1GQI
Structure of Pseudomonas cellulosa alpha-D-glucuronidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-13 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 69.912, 74.682, 87.587 |
Unit cell angles | 115.18, 93.14, 109.33 |
Refinement procedure
Resolution | 20.000 - 1.480 |
R-factor | 0.133 |
Rwork | 0.133 |
R-free | 0.16800 |
Structure solution method | SIRAS |
RMSD bond length | 0.016 |
RMSD bond angle | 1.589 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.560 |
High resolution limit [Å] | 1.480 * | 1.480 |
Rmerge | 0.073 | 0.345 |
Number of reflections | 236943 * | 32167 * |
<I/σ(I)> | 7.1 | 1.6 |
Completeness [%] | 97.6 | 90.4 |
Redundancy | 4.2 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 20 * | 30MG/ML, 15% PEG3350, 250MM MGCL2, 5MM TRIS PH8.0, 20% ETHYLENE GLYCOL, pH 8.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
3 | 1 | reservoir | 250 (mM) | ||
4 | 1 | reservoir | ethylene glycol | 20 (%(v/v)) | |
5 | 1 | reservoir | PEG3350 | 15 (%(w/v)) |