1GQA
Cytochrome c' from Rhodobacter Spheriodes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 31 |
| Unit cell lengths | 48.100, 48.100, 115.798 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.000 * - 1.800 |
| R-factor | 0.20948 |
| Rwork | 0.204 |
| R-free | 0.25400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cpq |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 1.745 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 * | 0.160 * |
| Total number of observations | 67416 * | |
| Number of reflections | 24077 | 2553 * |
| <I/σ(I)> | 8.1 | 4.6 |
| Completeness [%] | 87.0 * | 57 * |
| Redundancy | 2.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 12MG/ML, 5+5 UL HANGING DROP, NA-ACETATE 25MM PH4.5, PEG 8000 15%, pH 4.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 50 (mM) | pH4.5 |
| 3 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
| 4 | 1 | reservoir | sodium phosphate | 50 (mM) | pH4.5 |
