1GQ7
PROCLAVAMINATE AMIDINO HYDROLASE FROM STREPTOMYCES CLAVULIGERUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.922, 81.348, 120.389 |
| Unit cell angles | 90.00, 99.55, 90.00 |
Refinement procedure
| Resolution | 35.590 - 2.450 |
| R-factor | 0.22 * |
| Rwork | 0.222 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gq6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.580 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.089 | 0.321 |
| Total number of observations | 262831 * | |
| Number of reflections | 65708 | 8947 * |
| <I/σ(I)> | 7.1 | 2.3 |
| Completeness [%] | 98.5 | 98.5 * |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 17 * | 24% PEG 400, 100 MM HEPES PH7.5, pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | 3-fold molar excess | ||
| 3 | 1 | reservoir | PEG400 | 24 (%(v/v)) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 5 | 1 | reservoir | 0.2 (M) |
