1GQ5
Structural Determinants of the NHERF Interaction with beta2-AR and PDGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-03 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.073, 50.073, 66.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.400 - 2.200 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i92 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.400 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 4966 | |
| <I/σ(I)> | 11.8 | 2.9 |
| Completeness [%] | 95.1 | 85.6 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | SODIUM ACETATE, SODIUM CHLORIDE, pH 4.60 |
