1GPY
CRYSTALLOGRAPHIC BINDING STUDIES ON THE ALLOSTERIC INHIBITOR GLUCOSE-6-PHOSPHATE TO T STATE GLYCOGEN PHOSPHORYLASE B
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.500, 128.500, 116.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.300* |
| R-factor | 0.203 |
| Rwork | 0.203 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 4.000 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.073 * |
| Total number of observations | 90321 * |
| Number of reflections | 35718 * |
| Completeness [%] | 78.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | glycogen phosphorylase b | 20-40 (mg/ml) | |
| 2 | 1 | 1 | IMP | 1 (mM) | |
| 3 | 1 | 1 | spermine | 1 (mM) | can be replaced with 10mM magnesium acetate |
| 4 | 1 | 1 | BES | 10 (mM) | |
| 5 | 1 | 1 | EDTA | 0.1 (mM) | |
| 6 | 1 | 1 | sodium azide | 0.02 (%(w/v)) |
