1GPM
ESCHERICHIA COLI GMP SYNTHETASE COMPLEXED WITH AMP AND PYROPHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 277 |
| Collection date | 1993-03-15 |
| Detector | WEISSENBERG |
| Wavelength(s) | 0.9100, 0.9804 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 156.000, 102.000, 78.800 |
| Unit cell angles | 90.00, 96.70, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.000 * |
| Data reduction software | XDS (MODIFIED FOR USE WITH SDMS) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.200 | |
| Rmerge | 0.059 * | 0.149 * |
| Number of reflections | 105224 | |
| Completeness [%] | 841.4 * | 48.4 * |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 | 20 * | pH 5.0 |
| 2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | GMPS | 8.5 (mg/ml) | |
| 10 | 1 | reservoir | PEG8000 | 10 (%) | |
| 2 | 1 | drop | sodium citrate | 34 (mM) | |
| 3 | 1 | drop | 3 (mM) | ||
| 4 | 1 | drop | ATP | 0.75 (mM) | |
| 5 | 1 | drop | 5'-monophosphate | 0.75 (mM) | |
| 6 | 1 | drop | DTE | 5 (mM) | |
| 7 | 1 | drop | EDTA | 1 (mM) | |
| 8 | 1 | drop | PEG8000 | 2.0-2.67 (%) | |
| 9 | 1 | reservoir | sodium citrate | 50 (mM) |
