1GP2
G PROTEIN HETEROTRIMER GI_ALPHA_1 BETA_1 GAMMA_2 WITH GDP BOUND
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1995-03 |
| Spacegroup name | P 43 |
| Unit cell lengths | 84.289, 84.289, 132.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.30700 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 | 0.222 |
| Number of reflections | 36822 | |
| <I/σ(I)> | 17 | 2.23 |
| Completeness [%] | 89.8 | 62 |
| Redundancy | 2.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 21 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 18 (%) | |
| 2 | 1 | reservoir | Na-HEPES | 100 (mM) | pH7.0 |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | pH6.4 |
| 4 | 1 | reservoir | n-beta-octyl-glucoside | 0.05 (%) | |
| 5 | 1 | reservoir | 2-propanol | 2 (%) | |
| 6 | 1 | drop | protein | 7.5 (mg/ml) |
