1GP0

Human IGF2R domain 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-15
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	49.405, 49.405, 118.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.620 - 1.400
R-factor	0.209
Rwork	0.209
R-free	0.23700
Structure solution method	OTHER
RMSD bond length	0.013
RMSD bond angle	1.662 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.097	0.452
Total number of observations	761896 *
Number of reflections	29550
<I/σ(I)>	35.7	9.4
Completeness [%]	98.7	95.6
Redundancy	25.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	3 *		PROTEIN WAS CRYSTALLIZED FROM 0.2 M LITHIUM SULFATE, 0.1 M TRIS HCL PH 8.5, 30 % PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	100 (mM)	pH3.0
3	1	drop		100 (mM)
4	1	reservoir	lithium sulfate	0.2 (M)
5	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
6	1	reservoir	PEG4000	30 (%)