1GOY

HYDROLASE(ENDORIBONUCLEASE)RIBONUCLEASE BI(G SPECIFIC ENDONUCLEASE) (E.C.3.1.27.-) COMPLEXED WITH GUANOSINE-3'-PHOSPHATE (3'-GMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	287
Detector	SYTNEX P21
Spacegroup name	P 21 21 21
Unit cell lengths	111.380, 69.560, 33.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.900 * - 2.000
R-factor	0.184 *
Rwork	0.184
Structure solution method	MIR
RMSD bond length	0.019 *
RMSD bond angle	2.200 *
Phasing software	BLANC
Refinement software	REFMAC (5.1.00)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.900 *
High resolution limit [Å]	2.000	2.000 *
Total number of observations	15152 *
Number of reflections	15152 *
Completeness [%]	82.9 *	34.2 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7		pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12-13 (mg/ml)
2	1	drop	PEG12000	12 (%)
3	1	drop	phosphate	25 (mM)	pH7.0
4	1	drop	ammonium sulfate	3 (%sat)
5	1	reservoir	PEG12000	23-24 (%)
6	1	reservoir	glycerol	50 (mM)	pH7.0