1GOW
BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 169.400, 169.400, 98.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26000 * |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.919 |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.057 |
| Number of reflections | 47968 |
| Completeness [%] | 96.9 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | beta-galactosidase | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 12.5 (mM) | |
| 3 | 1 | drop | PEG4000 | 7 (%(w/v)) | |
| 4 | 1 | drop | 75-100 (mM) | ||
| 5 | 1 | drop | acetate | 25-50 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
| 7 | 1 | reservoir | 150-200 (mM) | ||
| 8 | 1 | reservoir | acetate | 50-100 (mM) |
