1GOU
Ribonuclease Binase (G Specific Endonuclease) Unliganded Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Temperature [K] | 287 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.030, 33.290, 78.700 |
| Unit cell angles | 90.00, 119.08, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.650 |
| R-factor | 0.14 * |
| Rwork | 0.140 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BLANC |
| Refinement software | REFMAC (5.1.00) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | |
| High resolution limit [Å] | 1.650 | 1.650 * |
| Rmerge | 0.039 * | 0.130 * |
| Total number of observations | 95418 * | |
| Number of reflections | 30591 * | |
| Completeness [%] | 96.0 * | 94.1 * |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | pH 9.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12-13 (mg/ml) | |
| 2 | 1 | drop | PEG12000 | 12 (%) | |
| 3 | 1 | drop | phosphate | 25 (mM) | pH7.0 |
| 4 | 1 | drop | ammonium sulfate | 3 (%sat) | |
| 5 | 1 | reservoir | PEG12000 | 23-24 (%) | |
| 6 | 1 | reservoir | glycerol | 50 (mM) | pH7.0 |
