1GOQ
Thermostable xylanase I from Thermoascus aurantiacus - Room temperature xylobiose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | R-AXIS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.060, 59.560, 51.130 |
Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
Resolution | 5.000 - 1.800 |
R-factor | 0.167 |
Rwork | 0.167 |
R-free | 0.21200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1gok |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.170 | 0.338 |
Number of reflections | 20709 | |
Completeness [%] | 86.0 | 37.9 |
Redundancy | 6.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | HANGING DROPS CONTAINING 1:1 RATIO OF 20-30 MG/ML PROTEIN SOLUTION AND RESERVOIR SOLUTION (12 % TO 25 % PEG 6,000). THE CRYSTAL WAS SOAKED IN 0.5 M XYLOBIOSE, pH 7.00 |