1GOB
COOPERATIVE STABILIZATION OF ESCHERICHIA COLI RIBONUCLEASE HI BY INSERTION OF GLY-80B AND GLY-77-> ALA SUBSTITUTION
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.350, 86.800, 35.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.191 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 * |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9 * | 20 * | used to seeding, Ishikawa, K., (1993) Protein Eng., 6, 85. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-4 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 200 (mM) |
