1GO7

The metzincin's methionine: PrtC M226C-E189K double mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-08-15
Detector	MSC
Spacegroup name	P 31 2 1
Unit cell lengths	102.700, 102.700, 121.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.220 - 2.100
Rwork	0.172
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PRTC WILDTYPE
RMSD bond length	0.013
RMSD bond angle	1.759
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.0.36)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.098	0.553
Number of reflections	43655
<I/σ(I)>	12.5	3.2
Completeness [%]	100.0	100
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		PH 6.5, NACL