1GO3
Structure of an archeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-15 |
| Spacegroup name | P 43 |
| Unit cell lengths | 92.391, 92.391, 91.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.750 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.23800 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 * | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.092 | 0.234 |
| Number of reflections | 76814 | |
| <I/σ(I)> | 4.1 | 3 |
| Completeness [%] | 99.8 | 99.6 * |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 4 * | 100 MM TRIS/ HCL 1.3-1.4 M AMMONIUM DIHYDROGEN MONOPHOSPHATE, pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 20 (mM) | pH6.5 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 4 | 1 | drop | protein | 20-25 (mg/ml) | |
| 5 | 1 | reservoir | 1.3-1.6 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH4.6 |
