1GO2
Structure of Ferredoxin-NADP+ Reductase with Lys 72 replaced by Glu (K72E)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE D2AM |
Synchrotron site | ESRF |
Beamline | D2AM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-15 |
Spacegroup name | P 65 |
Unit cell lengths | 86.640, 86.640, 96.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1que |
RMSD bond length | 0.008 |
RMSD bond angle | 0.858 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.190 | 1.790 |
High resolution limit [Å] | 1.700 | 1.710 |
Rmerge | 0.059 | 0.340 |
Number of reflections | 43287 | |
<I/σ(I)> | 10.5 | 2.2 |
Completeness [%] | 98.0 | 98 |
Redundancy | 4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | pH 5.00 |