1GNY
xylan-binding module CBM15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-15 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.150, 60.122, 33.107 |
| Unit cell angles | 90.00, 102.78, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.630 |
| R-factor | 0.15 * |
| Rwork | 0.150 |
| R-free | 0.18000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIAL MODEL (UNPUBLISHED) |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 1.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.043 | 0.330 |
| Number of reflections | 21535 | |
| <I/σ(I)> | 29 | 3 |
| Completeness [%] | 98.0 | 80 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 0.2M NH4OAC,0.1M NA CITRATE PH 5.6, 30% POLYETHYLENE GLYCOL 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.2 (M) | ||
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | xylohexaose | 10 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |
