1GNU
GABA(A) receptor associated protein GABARAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.013, 55.124, 64.418 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.000 * - 1.750 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eo6 |
RMSD bond length | 0.025 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.065 | 0.247 |
Total number of observations | 82560 * | |
Number of reflections | 11007 | 1540 * |
<I/σ(I)> | 5.3 | 2.2 |
Completeness [%] | 97.6 | 95.1 |
Redundancy | 7.6 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 22 * | 18MG/ML GABARAP, 50MM TRIS PH8.5, 0.25M NACL,10%(W/V) PEGMONO, pH 8.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 36 (mg/ml) | |
2 | 1 | drop | Tris | 10 (mM) | |
3 | 1 | drop | 0.5 (M) | pH7.0 | |
4 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
5 | 1 | reservoir | PEG2000MME | 20 (%(w/v)) | |
6 | 1 | reservoir | nickel(II) chloride hexahydrate | 10 (mM) |