1GNR

X-RAY CRYSTAL STRUCTURE ANALYSIS OF THE CATALYTIC DOMAIN OF THE ONCOGENE PRODUCT P21H-RAS COMPLEXED WITH CAGED GTP AND MANT DGPPNHP

Experimental procedure

Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	1992
Detector	XENTRONICS
Spacegroup name	P 32 2 1
Unit cell lengths	40.400, 40.400, 158.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.850
R-factor	0.227 *
Rwork	0.206
R-free	0.25700
RMSD bond length	0.008
RMSD bond angle	1.451 *
Data reduction software	XDS
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.074 *
Total number of observations	43753 *
Number of reflections	13998
Completeness [%]	80.8 *
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.6 *		used to seed *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	PEG400	27 (%(v/v))
3	1	reservoir	Tris-HCl	64 (mM)
4	1	reservoir		10 (mM)
5	1	reservoir	DTT	20 (mM)
6	1	reservoir	PEG400	30 (%(v/v))