1GNK

GLNK, A SIGNAL PROTEIN FROM E. COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-11-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 3
Unit cell lengths	85.530, 85.530, 85.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.189
Rwork	0.189
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.664
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.01)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.047	0.182
Total number of observations	97474 *
Number of reflections	14384
Completeness [%]	99.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	277 *	Macpherson, K.H.R., (1998) Acta Crystallog. sect., D54, 996. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-13.3 (mg/ml)
2	1	reservoir	MPD	30 (%)
3	1	reservoir	ammonium acetate	0.2 (M)
4	1	reservoir	cacodylate	0.1 (M)	pH6.5