1GNK
GLNK, A SIGNAL PROTEIN FROM E. COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-11-15 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 3 |
Unit cell lengths | 85.530, 85.530, 85.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.664 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.01) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.047 | 0.182 |
Total number of observations | 97474 * | |
Number of reflections | 14384 | |
Completeness [%] | 99.8 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 277 * | Macpherson, K.H.R., (1998) Acta Crystallog. sect., D54, 996. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-13.3 (mg/ml) | |
2 | 1 | reservoir | MPD | 30 (%) | |
3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
4 | 1 | reservoir | cacodylate | 0.1 (M) | pH6.5 |