1GNI
HUMAN SERUM ALBUMIN COMPLEXED WITH cis-9-OCTADECENOIC ACID (OLEIC ACID)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2000-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 188.386, 38.923, 95.477 |
| Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
| Resolution | 32.800 - 2.400 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.800 * | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.062 | 0.372 |
| Number of reflections | 25527 | |
| <I/σ(I)> | 4.2 | 1.8 |
| Completeness [%] | 95.2 * | 99 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 4 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 100 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 36-37 (%(w/v)) | |
| 3 | 1 | reservoir | potassium phosphate | 50 (mM) | pH7.5 |
