1GN1
crystal structure of the mouse CCT gamma apical domain (monoclinic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.244, 234.229, 62.703 |
| Unit cell angles | 90.00, 114.70, 90.00 |
Refinement procedure
| Resolution | 29.110 - 2.800 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.28700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gml |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 * | 2.940 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.051 | 0.147 |
| Number of reflections | 33738 * | |
| <I/σ(I)> | 6.3 | 4.7 |
| Completeness [%] | 85.2 | 74.1 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.5 | 14 * | CRYSTALS GROWN BY MICROBATCH METHOD (UNDER OIL) USING A 1:1 MIXTURE OF 30MG/ML PROTEIN, 400MM NACL, 20% GLYCEROL, 8MM TRIS PH8.0, 0.4MM EDTA AND 14% PEG 8K, 100MM NA-CACODYLATE PH6.5, 40MM CA(OAC)2, 40% GLYCEROL, pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 30 (mg/ml) | |
| 2 | 1 | 1 | Tris | 8 (mM) | pH8.0 |
| 3 | 1 | 1 | 400 (mM) | ||
| 4 | 1 | 1 | EDTA | 0.4 (mM) | |
| 5 | 1 | 1 | glycerol | 20 (%) | |
| 6 | 1 | 1 | sodium cacodylate | 100 (mM) | pH6.5 |
| 7 | 1 | 1 | PEG8000 | 14 (%(w/v)) | |
| 8 | 1 | 1 | 40 (mM) | ||
| 9 | 1 | 1 | glycerol | 40 (%) |
