1GN0
Escherichia coli GlpE sulfurtransferase soaked with KCN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Spacegroup name | P 32 |
Unit cell lengths | 53.796, 53.796, 30.346 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.177 * |
Rwork | 0.173 |
R-free | 0.24800 * |
Structure solution method | OTHER |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.032 | 0.234 |
Total number of observations | 64419 * | |
Number of reflections | 9094 | |
<I/σ(I)> | 23 | 2.5 |
Completeness [%] | 95.7 * | 84.7 * |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.0 |
3 | 1 | drop | EDTA | 3 (mM) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | glycerol | 10 (%(v/v)) | |
6 | 1 | reservoir | 0.1 (M) | ||
7 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
8 | 1 | reservoir | 2-propanol | 20 (%(v/v)) |