1GMX

Escherichia coli GlpE sulfurtransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Collection date	2000-10-15
Spacegroup name	P 32
Unit cell lengths	53.870, 53.870, 30.523
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.000 - 1.060 *
R-factor	0.128 *
Rwork	0.128
R-free	0.15100 *
Structure solution method	SIRAS
RMSD bond length	0.018 *
RMSD bond angle	2.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.080
High resolution limit [Å]	1.060	1.060
Rmerge	0.023	0.281
Total number of observations	555366 *
Number of reflections	45040
<I/σ(I)>	43	3
Completeness [%]	99.6	93.6
Redundancy	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.0
3	1	drop	EDTA	3 (mM)
4	1	drop		100 (mM)
5	1	drop	glycerol	10 (%(v/v))
6	1	reservoir		0.1 (M)
7	1	reservoir	sodium acetate	0.1 (M)	pH4.6
8	1	reservoir	2-propanol	20 (%(v/v))