1GMX
Escherichia coli GlpE sulfurtransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Collection date | 2000-10-15 |
Spacegroup name | P 32 |
Unit cell lengths | 53.870, 53.870, 30.523 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.000 - 1.060 * |
R-factor | 0.128 * |
Rwork | 0.128 |
R-free | 0.15100 * |
Structure solution method | SIRAS |
RMSD bond length | 0.018 * |
RMSD bond angle | 2.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.080 |
High resolution limit [Å] | 1.060 | 1.060 |
Rmerge | 0.023 | 0.281 |
Total number of observations | 555366 * | |
Number of reflections | 45040 | |
<I/σ(I)> | 43 | 3 |
Completeness [%] | 99.6 | 93.6 |
Redundancy | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.0 |
3 | 1 | drop | EDTA | 3 (mM) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | glycerol | 10 (%(v/v)) | |
6 | 1 | reservoir | 0.1 (M) | ||
7 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
8 | 1 | reservoir | 2-propanol | 20 (%(v/v)) |