1GMM
Carbohydrate binding module CBM6 from xylanase U Clostridium thermocellum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-15 |
| Wavelength(s) | 0.9793, 0.9795, 0.9465, 0.933 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 59.650, 59.650, 157.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.000 * - 2.100* |
| R-factor | 0.2 * |
| Rwork | 0.208 |
| R-free | 0.21600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.550 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.220 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 * | 0.204 |
| Number of reflections | 11981 | |
| <I/σ(I)> | 9.3 | 1.2 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 6.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 4 * | 30% PEG 4000, 100MM NA-CITRATE PH 5.5, 100MM (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 20 (mM) | pH5.0 |
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
| 5 | 1 | reservoir | ammonium sulfate | 100 (mM) |
