1GMJ
The structure of bovine IF1, the regulatory subunit of mitochondrial F-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.010, 53.290, 156.940 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 26.000 - 2.200 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.28000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 15.050 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.200 | 2.350 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 | 0.227 |
| Number of reflections | 18750 | |
| <I/σ(I)> | 10.5 | 3.7 |
| Completeness [%] | 72.3 | 10.8 |
| Redundancy | 2.4 | 2. * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 23 * | CRYSTALS WERE GROWN BY EQUILIBRATING A IF1-H49K SOLUTION, AT 6 MG/ML IN BUFFER 10 MM TRIS-HCL PH 8.0, RESERVOIR CONTAINING 0.8 M MONO-SODIUM DIHYDROGEN PHOSPHAT AGAINST A 0.8 M MONO-POTASSIUM DIHYDROGEN PHOSPHATE AND 0.1 M HEPES-NA BUFFER PH 8, AT 23C, IN SITTING-DROP VAPOR-DIFFUSION TRAYS. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 3 | 1 | reservoir | mono-sodium dihydrogen phosphate | 0.8 (M) | |
| 4 | 1 | reservoir | mono-potassium dihydrogen phosphate | 0.8 (M) | |
| 5 | 1 | reservoir | HEPES-Na | 0.1 (M) | pH8. |
