1GMG

ALANINE 31 PROLINE MUTANT OF ROP PROTEIN, MONOCLINIC FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-11-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	94.390, 24.250, 64.530
Unit cell angles	90.00, 130.40, 90.00

Refinement procedure

Resolution	36.000 - 1.900
R-factor	0.187
Rwork	0.187
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	POLY-ALANINE MODEL OF ONE HELIX (RESIDUES 4-29) FROM PDB ENTRY 1RPO
RMSD bond length	0.005
RMSD bond angle	0.864
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	Queen of Spades
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.075	0.291
Number of reflections	8771
<I/σ(I)>	12.4	4.1
Completeness [%]	96.4	91.6
Redundancy	8.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	4.8		Glykos, N.M., (1999) Acta Cryst., D55, 1301. *