1GMC

THE X-RAY CRYSTAL STRUCTURE OF THE TETRAHEDRAL INTERMEDIATE OF GAMMA-CHYMOTRYPSIN IN HEXANE

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	69.540, 69.520, 98.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 2.200
R-factor	0.176
Rwork	0.176
RMSD bond length	0.011
RMSD bond angle	1.950
Refinement software	X-PLOR (1GMC)

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.064 *
Total number of observations	16994 *
Number of reflections	8058 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.6 *		referred to 'Stoddard, B. L.', (1990) Biochemistry, 29, 4871-4879 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	chymotrypsin	15 (mg/ml)
2	1	1	sodium cacodylate	10 (mM)
3	1	1	cetyltrimethylammonium bromide	0.75 (%sat)
4	1	1	ammonium sulfate	45 (%sat)
5	1	2	ammonium sulphate	65 (%sat)