1GM6
3-D STRUCTURE OF A SALIVARY LIPOCALIN FROM BOAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2001-01-15 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.112, 70.112, 71.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.130 |
| R-factor | 0.252 * |
| Rwork | 0.254 |
| R-free | 0.28300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ew3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 * | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.056 | 0.295 * |
| Number of reflections | 10884 | |
| <I/σ(I)> | 7.3 | 3 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | 1.95M AS, 0.1M NACI PH 5.5, 0.2M K/NA TARTRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.95 (M) | |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.5 |
| 3 | 1 | reservoir | potassium/sodium tartrate | 0.2 (M) | |
| 4 | 1 | drop | protein | 3 (mg/ml) | |
| 5 | 1 | drop | 10 (mM) |
