1GLV

THREE-DIMENSIONAL STRUCTURE OF THE GLUTATHIONE SYNTHETASE FROM ESCHERICHIA COLI B AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 62 2 2
Unit cell lengths	87.700, 87.700, 169.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.700
R-factor	0.177
RMSD bond length	0.018
RMSD bond angle	0.041 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.033 *
Total number of observations	32629 *
Number of reflections	10757 *
Completeness [%]	98.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	5.5 *	25 *	referred to J.Mol.Biol. 209.503-504 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
2	1	1	glutathione synthetase	2 (%(w/v))
3	1	1	ammonium sulfate	20 (%sat)
4	1	1		5 (mM)
5	1	1	potassium phosphate	50 (mM)
6	1	2	ammonium sulfate	27 (%sat)
7	1	2		5 (mM)
8	1	2		0.02 (%(w/v))
9	1	2	potassium phosphate	50 (mM)