1GLN
ARCHITECTURES OF CLASS-DEFINING AND SPECIFIC DOMAINS OF GLUTAMYL-TRNA SYNTHETASE
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.750, 110.090, 67.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.039 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 6.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.042 * |
| Number of reflections | 13867 |
| Completeness [%] | 90.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | Nureki, O., (1992) Eur. J. Biochem., 204, 465. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 2 | 1 | reservoir | 2-mercaptoethanol | 10 (mM) |
