1GLH

CATION BINDING TO A BACILLUS (1,3-1,4)-BETA-GLUCANASE. GEOMETRY, AFFINITY AND EFFECT ON PROTEIN STABILITY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	70.220, 72.560, 49.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.198
R-free	0.22500
RMSD bond length	0.015
RMSD bond angle	0.047
Refinement software	PROFFT

Data quality characteristics

	Overall
Number of reflections	13475
Completeness [%]	80.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		Keitel, T., (1991) J. Mol. Biol, 218, 703. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	70 (mg/ml)
2	1	drop	2-(N-morpholino)ethanesulfonic acid	20 (mM)
3	1	drop		0.02 (%(w/v))
4	1	reservoir	2-(N-morpholino)ethanesulfonic acid	20 (mM)
5	1	reservoir		0.02 (%(w/v))
6	1	reservoir	PEG6000	13 (%(w/v))