1GL6
Plasmid coupling protein TrwB in complex with the non-hydrolysable GTP analogue GDPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 151.600, 151.600, 252.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e9r |
RMSD bond length | 0.009 |
RMSD bond angle | 1.420 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.093 | 0.453 |
Number of reflections | 81160 | |
<I/σ(I)> | 6.1 | 1.7 |
Completeness [%] | 98.0 | 92 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.50 |