1GL4
Nidogen-1 G2/Perlecan IG3 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-06 |
| Detector | QUANTUM4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.920, 72.300, 103.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21 * |
| Rwork | 0.217 |
| R-free | 0.24500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h4u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.089 | 0.256 |
| Number of reflections | 29096 | |
| <I/σ(I)> | 11.4 | 4.7 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 10% PEG 6000, 5% MPD, 0.1 M HEPES PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | ammonium acetate | 0.04 (M) | pH6.8 |
| 3 | 1 | reservoir | PEG6000 | 10 (%) | |
| 4 | 1 | reservoir | MPD | 5 (%) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
