1GL2
Crystal structure of an endosomal SNARE core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS M18X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.700, 41.100, 51.500 |
| Unit cell angles | 90.00, 109.40, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 13.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.035 | 0.137 |
| Total number of observations | 71259 * | |
| Number of reflections | 14969 * | |
| <I/σ(I)> | 30.57 | 11.35 |
| Completeness [%] | 93.7 | 88.8 |
| Redundancy | 5.5 | 3.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 20 * | PROTEIN SOLUTION: 20 MG/ML OF QUATERNARY COMPLEX IN 20MM TRIS-HCL AT PH 7.4 WELL SOLUTION: 0.1M NA-ACETATE AT PH 5.2, 2.5 M NA-FORMIAT, 15% GLYCEROL HANGING DROPS WITH 2MUL + 2 MUL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.4 |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.2 |
| 4 | 1 | reservoir | sodium formate | 2.5 (M) | |
| 5 | 1 | reservoir | glycerol | 15 (%(v/v)) |
